Rapid detection of viable Bacillus anthracis in environmental samples using engineered reporter phages
نویسندگان
چکیده
3 Natasha J. Sharp, Ian J. Molineux, Martin A. Page, and David A. Schofield*, 4 5 Guild BioSciences, Charleston, South Carolina, 29407 6 Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas, 7 78712 8 United States Army Corps of Engineers, Engineer Research and Development Center, Construction 9 Engineering Research Laboratory, Champaign, Illinois, 61826 10 11 Corresponding author. David A. Schofield, E-mail: [email protected] 12 13 Running title: Environmental detection of anthrax spores 14 15 AEM Accepted Manuscript Posted Online 12 February 2016 Appl. Environ. Microbiol. doi:10.1128/AEM.03772-15 Copyright © 2016, American Society for Microbiology. All Rights Reserved.
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Rapid Detection of Viable Bacillus anthracis Spores in Environmental Samples by Using Engineered Reporter Phages.
Bacillus anthracis, the causative agent of anthrax, was utilized as a bioterrorism agent in 2001 when spores were distributed via the U.S. postal system. In responding to this event, the Federal Bureau of Investigation used traditional bacterial culture viability assays to ascertain the extent of contamination of the postal facilities within 24 to 48 h of environmental sample acquisition. Here,...
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